Recent content by samzorz

  1. S

    Preparing an extraction buffer with PMSF and 2-mercaptoethanol

    Homework Statement We have a 100 mL of 10 mM Tris-HCl, 1 mM EDTA, pH = 7.4 We're supposed to add PMSF to a final concentration of 1 mM and 2-mercaptoethanol to a final concentration of 1 mM. The stock solution of PMSF is .10 M PMSF in absolute ethanol. The 2-mercaptoethanol is also in the...