Recent content by ScienceEnthusiast

We havent' been spinning them, nor filtering, I think we will look into it. We haven't tried omitting the incubation with secondaries yet, will propably try it on monday. We block our membranes with 3% milk + 1% BSA solution, which shouldn't interact with our secondaries, if I understand...

Hello, a problem from a newbie with western blots. I've been doing western blots for a few months now, and everything was going alright. But 2 weeks ago, all blots started coming out badly as in the photos. Before, neither we had those black dots ont the sides of membranes, or those black...

Hello, I'm a biology student, that hopes to find some help from amazing people of ot forum. Perhaps one day I will be ablo to help others aswell.