I did test out that theory, Borek. When I added 30ml of buffer to 30ml of sediment the volume was 55ml. So yes, the sediment does contain interstitial spaces which is filled by the buffer. Still doesn't account for the concentration being double. Sigh.
Hi Borek -
The sediment is collected with minimal pore water. Since sediment is not created equal in terms of size and dimension, the recognized protocol is to standardize the sediment by volume. It is collected in syringes at a volume of 30 ml.
I believe the issue may lie in the fact the...
I need some assistance. I am truly missing something very fundamental.
Background: I have a sodium sulphide stock solution with the conc. of 0.0429M. I prepare five standards through serial dilution (10,000; 5,000; 1,000; 500; 100uM). Buffer is added in a 1:1 with the standard - the...