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    Enzyme Kinetics: Measuring Inhibitory Effects and Determining Km and Vmax Values

    Thanks for your answer Sorry panicked a little. It is an enzyme kinetics practical with inhibitors fructose and urea. I have the data that tells me the amount fructose/glucose and I put it in my question. I have figured out a lot by myself but thanks for your answer.
  2. S

    Enzyme Kinetics: Measuring Inhibitory Effects and Determining Km and Vmax Values

    Somebody please help me. Practical: A calibration graph should be prepared by taking 0.0 (zero, blank), 1.0, 2.0 and 3.0 ml aliquots of an aqueous solution containing both 1.67 mM D-glucose and 1.67 mM D-fructose. Add distilled water to maket it 3 ml. Add 1 ml DNS reagent. Incubate 5 min...
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