Recent content by sunny bob

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    Deconvolution IRF from fluorescence decay curves

    Thanks you guys! I tried the FFT deconvolution method, in Origin, before. However, it didn't give satisfying result.
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    Deconvolution IRF from fluorescence decay curves

    Hi Rajini, By any chance, do you have or know any free source of program written for deconvolution using NLSF? I have seen a code in O' Connors's book on "Time-correlated single photon counting" but as Iam a beginner in programming, I fear if it might take ages to finish it. Do you...
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    Deconvolution IRF from fluorescence decay curves

    Thanks Rajini! That was a helpful discussion. Thanks again.
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    Deconvolution IRF from fluorescence decay curves

    Hi Rajini, Thanks for responding. I got your point. But I think I did not frame my question well. Let me try to put it again. I could be able to fit the exponential decay data with exponential function. However the concern is the deconvolution of the Instrument response function(IRF...
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    Deconvolution IRF from fluorescence decay curves

    Hi There, I have been working on fluorescence decays of the fluorophores whose lifetimes are comparable to the instrument response function(IRF) of my device. The technique I use is based on Time-correlated single photon Counting(TCSPC). I have been fitting the decay curves using...
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