Hi Rajini,
By any chance, do you have or know any free source of program written for deconvolution using NLSF? I have seen a code in O' Connors's book on "Time-correlated single photon counting" but as Iam a beginner in programming, I fear if it might take ages to finish it. Do you...
Hi Rajini,
Thanks for responding. I got your point. But I think I did not frame my question well. Let me try to put it again. I could be able to fit the exponential decay data with exponential function. However the concern is the deconvolution of the Instrument response function(IRF...
Hi There,
I have been working on fluorescence decays of the fluorophores whose lifetimes are comparable to the instrument response function(IRF) of my device. The technique I use is based on Time-correlated single photon Counting(TCSPC). I have been fitting the decay curves using...