Recent content by sunny bob
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Graduate Deconvolution IRF from fluorescence decay curves
Thanks you guys! I tried the FFT deconvolution method, in Origin, before. However, it didn't give satisfying result.- sunny bob
- Post #10
- Forum: Atomic and Condensed Matter
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Graduate Deconvolution IRF from fluorescence decay curves
Hi Rajini, By any chance, do you have or know any free source of program written for deconvolution using NLSF? I have seen a code in O' Connors's book on "Time-correlated single photon counting" but as Iam a beginner in programming, I fear if it might take ages to finish it. Do you...- sunny bob
- Post #6
- Forum: Atomic and Condensed Matter
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Graduate Deconvolution IRF from fluorescence decay curves
Thanks Rajini! That was a helpful discussion. Thanks again.- sunny bob
- Post #5
- Forum: Atomic and Condensed Matter
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Graduate Deconvolution IRF from fluorescence decay curves
Hi Rajini, Thanks for responding. I got your point. But I think I did not frame my question well. Let me try to put it again. I could be able to fit the exponential decay data with exponential function. However the concern is the deconvolution of the Instrument response function(IRF...- sunny bob
- Post #3
- Forum: Atomic and Condensed Matter
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Graduate Deconvolution IRF from fluorescence decay curves
Hi There, I have been working on fluorescence decays of the fluorophores whose lifetimes are comparable to the instrument response function(IRF) of my device. The technique I use is based on Time-correlated single photon Counting(TCSPC). I have been fitting the decay curves using...- sunny bob
- Thread
- Curves Decay Deconvolution Fluorescence
- Replies: 9
- Forum: Atomic and Condensed Matter