Recent content by tr111

cause what I like to build is so simple, I have to do this calculation first to make sure later on the intensity of fluorescence of my sample that hit on the camera could be saw by the CCD sensor.

I would like to build a fluorescence measurement setup as simple as possible,just like this one, http://www.scienceinyoureyes.com/uploads/pics/Fluorescent-microscopy_en.gif got the filters needed, and an objective lens, even one more convex lens for tube lens as in microscope but without any...

Hi, When we use an optical microscope, how do we roughly calculate the illuminated spot size on the sample ? Also what is the light power on that spot? And what if I change to fluorescence microscope(epi illumination)? Cause the illuminating light go through the rear side of objective to...