I would have to read more about it but I know it’s “real time PCR”. where does the derivative come to play exactly? And how does an inanimate dye fluoresce during denaturation?
Hello, PF I’m new here. Can someone please help me explain how to interpret thermal shift experiments work for binding? Apparently the data you receive from such experiment is a derivative dF/dT where F=fluorescence and T=temperature; and I’m very confused because isn’t the experiment supposed...