question on DNA gel analysis

[biochemist]

Hi everyone, I ran a gel for restriction digestion and used lamda HindIII as the marker. When I'm drawing the graph on a semi-log paper, size on y axis and distance from the well on the x axis, the standard curve isn't a straight line, but a linear curve. Is it fine?

Another question: the smallest digit given on a semi-log graph paper for DNA size is 1, but I've got a 0.5 band and couldn't point it out on the graph. What shall I do? Shall I just ignore this?

Very much appreciated if you could help me solve my problems

[biochemist]

I'm really confused, cos I've learnt that the distance from the well measured should be proportional to the log10 value of the DNA size. And by theory I should have got a straight line or nearly straight, but the curve I've got doesn't seem to be straight at all!

[biochemist]

Anyone can answer my question?