Recent content by asy

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    Stability and Storage Conditions for Common Solutions | Expert Tips

    I will need to make the following for determining enzyme activity. 0.1 M Hydroxylamine; 10 mM Glutathione; 30 mM CBZ-L-glutaminylglycine and I am trying to find out if I can make this a stock solution or if I have to make it fresh every time. Thank you
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    Stability and Storage Conditions for Common Solutions | Expert Tips

    :p thanks... but... Not always that easy!
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    Stability and Storage Conditions for Common Solutions | Expert Tips

    Hello everyone, I need to know the stability and storage conditions of some solutions that I need to prepare. Is there a place where I can find this information (at least for most common solutions)? Or is there any bibliography to consult? Thanks
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    How to make 0.2 M Tris-Acetate Buffer pH 6

    Thank you for the help!
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    How to make 0.2 M Tris-Acetate Buffer pH 6

    Thanks for the feedback. I'm trying to use a method that I found in the literature, which was cited in several papers. Folk JE and Cole PW (1966) The Journal of Biological Chemistry 241(23): 5518–5525 "Each test was made in a final volume of 0.5 ml of 0.2 M Tris-acetate buffer, pH 6.0...
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    How to make 0.2 M Tris-Acetate Buffer pH 6

    Hello, I need to make a 0.2 M Tris-Acetate Buffer pH 6 and I can't find a protocol for this specific buffer. I know that I can make a Tris solution (i.e. 12.11 g of tris(hydroxymethyl)aminomethane in water), adjust the pH to 8 and dilute to 1000 mL with water. Is the same true for pH 6...
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