I will need to make the following for determining enzyme activity.
0.1 M Hydroxylamine; 10 mM Glutathione; 30 mM CBZ-L-glutaminylglycine
and I am trying to find out if I can make this a stock solution or if I have to make it fresh every time.
Thank you
Hello everyone,
I need to know the stability and storage conditions of some solutions that I need to prepare. Is there a place where I can find this information (at least for most common solutions)? Or is there any bibliography to consult?
Thanks
Thanks for the feedback.
I'm trying to use a method that I found in the literature, which was cited in several papers.
Folk JE and Cole PW (1966) The Journal of Biological Chemistry 241(23): 5518–5525
"Each test was made in a final volume of 0.5 ml of 0.2 M Tris-acetate buffer, pH 6.0...
Hello,
I need to make a 0.2 M Tris-Acetate Buffer pH 6 and I can't find a protocol for this specific buffer.
I know that I can make a Tris solution (i.e. 12.11 g of tris(hydroxymethyl)aminomethane in water), adjust the pH to 8 and dilute to 1000 mL with water.
Is the same true for pH 6...