Recent content by Igor 77

Dear Snorkack, Thank you for this suggestion. Since our unknown membrane protein does not exchange atoms/groups with the transported substrate (homocitrate), I consider cross-linking radio-labeled homocitrate to all proteins which it encounters while on the way to its "gate" trough the...

Dear Mfb, dear Snorkack, Thank you for your informative replies. Unfortunately, for my application, it would be insufficient to visualize the interaction between homocitrate and its transport protein in the membrane. My goal is to tag the transport protein upon contact with its substrate, so...

Dear Mfb, Essentially, the substrate passes through a channel-like protein embedded into a lipid bi-layer (cell membrane). The substrate spends only very short time in the protein, but is physically very close to its surface. Also, more than one molecule passes through in a short period of...

Dear Gleem, dear Mfb, Thank you for your informative replies. Since in our system the transport protein does not chemically react with the transport substrate, we have no means to transfer the radiolabel from the substrate to the transporter. Currently I try to figure out if chemical...

Dear Forum Members, I am a molecular biologist. One of my projects is focused on the identification of a protein that interacts with a known organic molecule. Namely, I try to chase a transmembrane protein that is known to transport one organic acid. If possible, I would like to get an idea on...