hey thanks for ur posts. i understand now the 21.2 kbp is not the missing one. its becasue in an ecor1 digest of lambda it produce 6 fragments two of which are alomost the same length i.e the 5.6 and 5.8 kbp therefore we can only see 5 bands not 6 becasue the have migrated at the same pace to...
just want to know why if there are six fragments present in an Eco R1 digest of lambda then why are there only five bands visible on a .8% agarose gel? so where have the 21.2 kbp gone? is it to big to migrate therefore has not show up on the gel? can someone explain to me please? thankyou