Recent content by MatthewHaas

I am sorry for the late reply. No, nothing got out of the well. We have not rulerd out power supply issues.

Has anyone ever ran a CHEF gel before? I am having trouble getting my plugs to leave the wells. Ethidium bromide staining shows a thick band in the well, and I am supposed to have a ladder. I have some images I can post, but I figured it makes more sense to first see if anyone out there has any...

Thanks for the responses everyone. I run 96 samples at a time because this is a population genetics project and the number of reactions that I am performing numbers in the thousands. I've done quite a few more gels now, and some have bands clear as day on the edges. I am beginning to...

I need to run 96 samples at a time, otherwise I would consider smaller.

Hi everyone, I am running agarose gels (1.5%, 100 V, 100 min) with the product of PCR-RFLP. My gel has 96 wells...48 on top and 48 on the bottom, so it is a rather large gel...300 ml by volume. I am having trouble getting clear, bright bands for the DNA in the lanes near the edges. It...

Well, I quit my PhD, but for entirely different reasons. I got along great with the other students. We actually still keep in touch, and its been 10 months since I left. I left because I really didn't care about what I was doing and wanted to live someplace else. I don't regret it for a...

Usually all staff and faculty get 1 course free per semester. I know several professors who have taken classes-for fun, primarily.

I don't know if I want to work with plants...I just kind of randomly picked that area of focus and ran with it. It didn't "pick me" which would be a completely different scenario. As for travel, I'm much more interested in going to Europe and S America more so than Africa..Not that that has...

Thanks.

I can't start a family just "anywhere". I'm gay, so there aren't opportunities to meet people to date in a lot of places. That brings me to the 3rd point-I cannot be friends with people who don't support equal rights. I could care less about any other political issue, but at least in large...

I want to live in near family, and in a large city. Just my personal preference. Another degree in a more technical field will help land me a job in this area. EDIT: I thought I would add more to better explain myself: -Is it worth being alone every night, and every weekend for a...

I left Plant Pathology because most, if not all, of the jobs I would be eligible for are in places I don't want to live.

Hi all, So I graduated from a small liberal arts school in Minnesota with my B.A. in Biology last year (2009). I'm not really sure why, but I decided on Plant Pathology for graduate school, which landed me in a PhD program at NDSU in Fargo, ND. Now, what I really wanted to do was live in...

Thank you, Delphi51. That was very helpful! And painfully obvious now that I look at it. Any tips on how to "sense" these things? Or just practice?

A speeder traveling at a constant speed of 121 km/h races past a billboard. A patrol car pursues from rest with constant acceleration of (8.3 km/h)/s until it reaches its maximum speed of 201 km/h, which it maintains until it catches up with the speeder. (a) How long does it take the...