Recent content by MrGenetic

Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific for dna sequence like CAATTAGTCA... for example my book says; ''MITF's binding domain can attach just...

If -Nh2 terminal become Nh3+, is this bond formation release energy in terms of entalpy decreasing way?

When the aminoacid environment were changed with adding OH or etc., Aminoacid N terminal were affected by H amount and in determined pKa, they loose hydrogen from N terminal. NH3 goes Nh2. My question is related to this. While this event occur, how change dipole moment of this terminal? Electron...

While polar molecule solved in solution, is this refers to ionizable molecule? Is it necessary or is it different things according the definitions?

But, gene of interested don't have to goes in nuclear dna i think, it might stay also in cytoplasm instead of making crossover with nuclear dna. Just stay like a plasmid dna with no homolog sequence with nuclear Dna But, Transformation event can occur without forcing in nature. So why we need...

Books say 3 possibilities for this situation as insert within Lacz, insert outside Lacz, no inserted vectors, then these goes the transformation with Ecoli. But we can't know transformation yield %100. Thus, if we make blue white screening there is more possibilities. Lacz-- inserted within...

Hi. If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc.. But i have a trouble with one issue about that. Books say that ; We must choose the vector that specific according to the cell that will do transformation. But we will treat with...

Can torsion angles were affected by repulsive effect of aminoacid interactions between side chains? Can torsion angles were affected by hydrophilic or hydrophilic interactions between side chains? I know that protein levels were affected by them but torsion angles is suspicious for me

What is the main differences of RFLP, VNTR, SNT, SNP ?

why sp2 hyb. have more e. density comp with sp3 in nmr calculation stuff ?