- #1
MrGenetic
- 10
- 0
Hi.
If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc..
But i have a trouble with one issue about that. Books say that ; We must choose the vector that specific according to the cell that will do transformation. But we will treat with calcium chloride and ice, why we choose specific vector?, this foreign vector can be enforced the cell for accepting. I know cell might accept the vector as a homolog sequence and might do cross with this vector, might take homolog genes into own dna, but it doesn't have to be. Vector might stay in the cytoplasm and i think it don't require the homolog sequences with cell's own DNA
Transformation should occur spontaneously due to bacteria nature, already
If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc..
But i have a trouble with one issue about that. Books say that ; We must choose the vector that specific according to the cell that will do transformation. But we will treat with calcium chloride and ice, why we choose specific vector?, this foreign vector can be enforced the cell for accepting. I know cell might accept the vector as a homolog sequence and might do cross with this vector, might take homolog genes into own dna, but it doesn't have to be. Vector might stay in the cytoplasm and i think it don't require the homolog sequences with cell's own DNA
Transformation should occur spontaneously due to bacteria nature, already
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