Recent content by newlabguy

Hi guys, First of all, thank-you for helping me thus far. I found R for Mac OS X on that cran site and installed it. Turns out they have grofit as well and I was able to install the grofit package. But as a first time user, I'm a little confused on what to do now. I've opened that paper that...

gompertz Unfortunately, I don't have access to R. I want to fit it to a model instead of using regression because I want some biologically meaningful parameters to compare. Biologically, I'm trying to determine if the CpxR transcription factor is repressing an operon. I've created a cpxR...

Hello, I'm in need of someone to show me how to fit a bacterial growth curve to data in Prism, preferably using the Gompertz function. I also need someone to show me how to assess the goodness of this fit. I have many different growth curves and I need to compare the parameters. Hopefully...

I'm interested in coating a coverslip with a thin layer of silver for SERS. I intend to grow a salmonella biofilm on it afterwards and get the spectrum. Does anyone have any experience with SERS and if so can you give me your advice for preparing SERS substrates to adsorb biological materials...

Thank you very much for your replies. I'm still confused about the matter and I think it might be due to my error in interpretation of vibrational states. The set of eigenvalues associated with a one-dimensional harmonic oscillator for a diatomic molecule is Ev=hf(v+(1/2)) v=1,2,3...

I'm confused about infrared spectroscopy. Wikipedia states that "when the frequency of the IR is the same as the vibrational frequency of a bond, absorption occurs." However, if I look at some vibrational transitions of HCl for the anharmonic oscillator, I see: 0-->1 \omega = 2885.7...

Hello Group! I'm working on analyzing Raman spectra of proteins. I'm particularly interested in learning more about the signal processing of the amide I band. If anyone has any experience with Peakfit program or Origin, Savitsky-Golay smoothing, or amide I deconvolution for secondary...

This isn't homework. It's for my job. I'm supposed to be making a 50mM phosphate buffer to pH 7.2, 0.5 NaOH. I have access to salts, K2HPO4 (dibasic), KH2PO4 (monobasic), and NaCl. I've determined that I need two liters of buffer solution. I did the calculations but when I measured the pH after...