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Raman spectroscopy amide I processing

  1. Apr 4, 2010 #1
    Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
    particularly interested in learning more about the signal processing
    of the amide I band. If anyone has any experience with Peakfit program
    or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
    secondary structure quantification, please help. My boss asked me to
    learn Raman because he's interested in monitoring the change in
    secondary structure in complex coacervates between protein and
    polysaccharide. Hopefully, I can get someone to describe to me what
    steps are involved in deconvoluting the amide I band in either peakfit
    or origin and why those steps are taken.
     
  2. jcsd
  3. Jul 15, 2010 #2
    take some chemistry course or ask your boss to employ a chemist.
     
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