Hello Group! I'm working on analyzing Raman spectra of proteins. I'm particularly interested in learning more about the signal processing of the amide I band. If anyone has any experience with Peakfit program or Origin, Savitsky-Golay smoothing, or amide I deconvolution for secondary structure quantification, please help. My boss asked me to learn Raman because he's interested in monitoring the change in secondary structure in complex coacervates between protein and polysaccharide. Hopefully, I can get someone to describe to me what steps are involved in deconvoluting the amide I band in either peakfit or origin and why those steps are taken.