Raman spectroscopy amide I processing

In summary, the speaker is asking for help with analyzing Raman spectra of proteins and is specifically interested in the signal processing of the amide I band. They mention programs and techniques such as Peakfit, Origin, Savitsky-Golay smoothing, and amide I deconvolution for secondary structure quantification. They explain that their boss wants them to learn Raman to monitor changes in secondary structure in complex coacervates, and they are looking for someone to describe the steps involved in deconvoluting the amide I band and why those steps are necessary. They also suggest taking a chemistry course or hiring a chemist for assistance.
  • #1
10
0
Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.
 
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  • #2
newlabguy said:
Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.

take some chemistry course or ask your boss to employ a chemist.
 

What is Raman spectroscopy?

Raman spectroscopy is a technique used to study the vibrational energies of molecules by measuring the scattered light from a sample. This can provide information about the chemical structure and composition of a substance.

What is the amide I region in Raman spectroscopy?

The amide I region in Raman spectroscopy refers to the spectral range of approximately 1600-1700 cm-1, which is associated with the stretching vibrations of the C=O bond in proteins and other biomolecules.

How is amide I processing used in Raman spectroscopy?

Amide I processing is a method used to analyze and interpret the complex spectra in the amide I region. It involves applying mathematical algorithms to the raw data in order to extract meaningful information, such as the secondary structure of proteins.

What are some advantages of using Raman spectroscopy for amide I processing?

Raman spectroscopy is a non-destructive and label-free technique, meaning that it does not require any chemical modifications or sample preparation. It can also provide information about the structure and dynamics of biomolecules in their natural environment.

What are some potential applications of Raman spectroscopy amide I processing?

Raman spectroscopy amide I processing has a wide range of applications in fields such as biochemistry, biophysics, and pharmaceuticals. It can be used to study protein folding, protein-ligand interactions, and the effects of disease or mutations on protein structure.

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