Raman spectroscopy amide I processing

  • Thread starter newlabguy
  • Start date
  • #1
10
0

Main Question or Discussion Point

Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.
 

Answers and Replies

  • #2
117
0
Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.
take some chemistry course or ask your boss to employ a chemist.
 

Related Threads on Raman spectroscopy amide I processing

  • Last Post
Replies
5
Views
1K
  • Last Post
Replies
9
Views
5K
Replies
2
Views
2K
  • Last Post
Replies
3
Views
13K
  • Last Post
Replies
4
Views
11K
  • Last Post
Replies
1
Views
2K
  • Last Post
Replies
2
Views
5K
  • Last Post
Replies
1
Views
2K
  • Last Post
Replies
8
Views
3K
Top