Recent content by VirtualAgeJ

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    A question about Km's of Firefly Luciferase

    Dear Eshi, Your boss might be on to something as ATP plugs into the active on top of luciferin (LH2). If you enhance the affinity (remember the real measure of that is Ks - possible with quenched Trp fluorescence experiments) for LH2, you might upset the reaction for ATP if LH2 (added first in...