can lacZ work when .....

[indoubt]

if i insert lacZ into a gene and the translation gives a hybrid product with the sequences of both lacZ and this gene. i wonder if lacZ can still work and give blue colonies? and what about the function of this other gene?


wait so much for replies.


thanks so much in advance!

[iansmith]

LacZ should still work when fused with a protein. The other protein might be affected by the fussion. It will depend on where lacZ is fused. The N-terminal and/or the C-terminal of some proteins are essantial for their role. There numerous example in the litterature that have shown that lacZ can be fused to genes.

[indoubt]

many thanks iansmith!