Amino acid biosynthesis from CAC intermediates

[Jikx]

Hello,

I'm having a bit of trouble with an experiment. It involves converting furamate and citrate into amino-acids using a transaminase. We are using a liver homogenase, with the amino-donor being alanine.

However, whatever resource i use, furamate/citrate is not converted into an animo-acid!

Any suggestions? THANKS!

(my experiment failed, but I still to write up the discussion!)

[Jikx]

I worked it out.. kind of.

Citrate is converted to oxaloacetate (via CAC), then undergoes a transamination reaction to Aspartate.

Furamate is converted to a-ketoglutarate (also via CAC), then becomes Glutamate.

Its just a curse with me... always ignore the simplest answer because its too simple.