reducing or not!

[mountain]

what is the difference between a non-reducing and a reducing buffer? :uhh:

and what does it mean with non-reducing and reducing?

thanks a bunch!

[Monique]

Are you familiar with redox reactions? Reduction and Oxidation. The cytoplasm of a cell is a reducing environment: an effect is that disulfide bonds within proteins get reduced and are thus hard to form.

DTT is a reducing agent and often added to reducing buffers.

[mountain]

thanks Monique! it seems like you know alot of things :smile: are you a PhD?