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Oct7-04, 12:01 PM
Sci Advisor
PF Gold
iansmith's Avatar
P: 1,430
We usually add 5 uL to well and it is good enough for one run. I have see people in our lab add up 20 uL and used for 3-4 runs. I don't like adding a lot because EtBr is inactivated by light and has the stock get older, it is not as stable as a fresh stock.

The only material that you do not get contaminated will be the flask you used for pouring the agarose gel.