The discussion focuses on the relationship between the charge of macromolecules and their velocity and weight during gel electrophoresis. It highlights the need for a formula that incorporates time to calculate the charge, velocity, and mass of these molecules. The separation of molecules in electrophoresis is primarily based on their charge, which affects their movement through the gel. Understanding this relationship is crucial for accurate analysis in biochemical experiments. Overall, the inquiry seeks to establish a quantitative framework for analyzing macromolecule behavior in electrophoresis.