Testing photoheterotrophic, autotrophic and fermentation of R.rubrum?

prime-factor

I need to test photoheteretrophic, autotrophic and fermentation growth of R.rubrum bacteria, all of which require anaerobic conditions. For fermentation, i am placing the bacteria with fructose in the dark, and for phototrophic in the light with fructose, and for autotrophic in the light with nothing. How should I do this to get some quantitative data??

How do I get anaerobic conditions,and how should i test this?, please help

Ouabache

Have you considered serial dilution and spread plating?
Have you considered GasPak culture?

prime-factor

Thanks. Unfortunately our school has no equipment such as the GasPak you mentioned
However , the bacteria were put in test-tubes filled with sterile water, then covered with oil. Hopefully things will work out.