|Feb22-10, 09:02 PM||#1|
What kind of errors would be produced by "excess" taq in PCR?
Not sure if this belong in the Homework section. Put it here since it's not homework.
What kind of errors will I get by adding 6.75ÁL taq pol to +50ÁL of cDNA? The master mix will be added to the cDNA resulting in an excess of taq pol. Only 2 and 4ÁL of the taq+cDNA mixture will be used for each reaction not the entire +50ÁL.
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