Raman spectroscopy amide I processing

[newlabguy]

Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.

 

[mccoy1]

Hello Group! I'm working on analyzing Raman spectra of proteins. I'm
particularly interested in learning more about the signal processing
of the amide I band. If anyone has any experience with Peakfit program
or Origin, Savitsky-Golay smoothing, or amide I deconvolution for
secondary structure quantification, please help. My boss asked me to
learn Raman because he's interested in monitoring the change in
secondary structure in complex coacervates between protein and
polysaccharide. Hopefully, I can get someone to describe to me what
steps are involved in deconvoluting the amide I band in either peakfit
or origin and why those steps are taken.

take some chemistry course or ask your boss to employ a chemist.