Recent content by littledog

Is there any research about this?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did anyone has taken the research on control the protein phosphorylation in wildtype microorganisms with...

YES，I almost buy all the DNA dyes of Thermo Fisher.

But as what I observe in my experiment，there are a lot of bacteria in dividing phase couldn't be stained by SYTO13，and what I wonder is whether the DNA structure or I should say the density of DNA and its binding protein in dormant bacteria greatly effect the affinity between DNA and nucleic dye？

I have try some methods to increase membrane permeability, the result shows that few effect on staining efficiency to inactive bacteria.

I found that it's difficult to stain dormant bacteria or bacteria in lag phase with Nucleic acid dye like SYTO9/SYBR Green Ⅰ, does anyone know why? DNA 3D structure too complex? DNA binding protein too much? Low material transport efficiency in bacteria? Or anyother factors? Is there any...