What are the darker regions in annealed 1018 steel?

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Hi, steel 1018 usually has between 0.15-0.20 % of Carbone, yes, the darker regions are perlite
image002.jpg
 
Sure, dark structure is Perlite.
Carbon fraction is given in alloy specification

1018 [tex]\rightarrow[/tex] 0.18% Carbon

And ... Annealing Treatment ( Total Annealing) may result in perlite + ferrite.
It's shown in your micrograph
Dark - perlite
Bright - Ferrite