Calculating kPa/min in Fermentation: What's the Correct Method?

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SUMMARY

The correct method for calculating kPa/min in yeast fermentation involves measuring the change in pressure over time using a gas pressure sensor. The formula to determine the rate is the difference between the initial and final pressure readings divided by the time interval in minutes. For example, if the initial pressure is 99.05 kPa and the pressure after one minute is 99.03 kPa, the calculation yields a rate of -0.02 kPa/min. It is essential to use the most distant pressure readings for accuracy and to ensure that time is measured in minutes for the desired units.

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This discussion is beneficial for fermentation scientists, biochemists, and anyone involved in experimental design and data analysis related to yeast fermentation processes.

huskyrunner5
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How do you calculate kpa/min??

1. I am trying to find the rate of CO2 production in yeast fermentation. I used a gas pressure sensor to measure the kpa every minute. I recorded the initial kpa and the kpa after every minute for four minutes. I forgot how to calculate the kpa/min and need clarification



2. I think the equation is something like this:
Initial pressure - final pressure/ (seconds?) I doubt this is right



3. For instance, the initial pressure was 99.05 kpa and after a minute it was 99.03.
So would the rate be -.02 kPa/min?
Can someone please help or steer me in the right direction?
Any help would be appreciated.
 
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You plot a graph of pressure vs time and find the slope
(you have more than one data point so you may as well use them)

Quick-and-dirty method, take the difference between final and initial pressure readings, divide by 4mins.

For instance, the initial pressure was 99.05 kpa and after a minute it was 99.03.
So would the rate be -.02 kPa/min?
Yes - do you need to worry about uncertainty? Best to take values farthest apart: it's change in pressure divided by change in time.
Measure changes in the units you need - so if you want kPa/min, measure pressure in kPa and time in mins ... if you need psi/decade, then...
 


huskyrunner5 said:
For instance, the initial pressure was 99.05 kpa and after a minute it was 99.03.
So would the rate be -.02 kPa/min?

Yes, that's the average rate of the pressure change for the first minute.
 

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