Cloning & Transforming Bacterial Vector for Protein Production

[Raghav Gupta]

Homework Statement

Scientists isolated a clinically important protein producing gene from human cells and they want to clone it and transform into a bacterial cell, so that they can induce the protein synthesis and produce the protein commercially. But they are unable to get the protein even though all procedures are correct- they isolated the DNA then they amplified the desired gene and cloned into a bacterial vector and transformed into a bacterial cell. What may be the reason for protein is not getting produced in the bacterial cell.

Homework Equations

NA

The Attempt at a Solution

Is it that some plastmid is not able to recombine with particular gene and the same plasmid combine?

 

[Ygggdrasil]

Consider some of the differences between bacteria and eukaryotes. Do genes from one type of organism contain features that the other type of organism lacks?

 

[Raghav Gupta]

Consider some of the differences between bacteria and eukaryotes. Do genes from one type of organism contain features that the other type of organism lacks?

But I have read that scientists are able to produce insulin from bacteria by this cloning method.
There are many differences in bacteria and humans.
Human have a large size DNA and bacteria have small size.

 

[Ygggdrasil]

Yes, it is possible to express human proteins in bacteria, but there are important changes that you must make if you are starting from the gene sequence (hint: you do not need to make these changes if you are starting from the mRNA sequence). Review the basic steps of transcription in bacteria and in eukaryotes, and you are likely to come across the answer.