Determining mutated, mRNA, Amino Acid sequence

[sonyab523]

I was wondering if I did something wrong with the deletion, it seems pretty self explanatory but I just want to make sure I did this right. The filled in answers are in bold And I'm completely lost with the Amino Acid sequence, my TA did this in class and I still don't get it.


Sequence of DNA template: 3'- TAC TGG CCG TTA GTT GAT ATA ACT-5'
Nucleotide number --> 1__________________________________23

Mutant 2: A one-nucleotide deletion at nucleotide 7

Original sequence: 3'-TAC TGG CCG TTA GRR GAT ATA ACT-5'

Mutated Sequence: TAC TGG CG TTA GRR GAT ATA ACT

mRNA Sequence: AUG ACC GC AAU CAA CUA UAU UGA

Amino Acid Sequence: met thr ? ser gln lev tyr

 

[TeethWhitener]

The deletion is far more devastating to the amino acid sequence than that. You need to regroup the mRNA bases in threes after the deletion:
mRNA Sequence: AUG ACC GCA AUC AAC UAU AUU GA
Which gives an amino acid sequence of:
Met Thr Ala Ile Asn Tyr Ile
with the GA left over. If this were part of a longer mRNA strand, the ribosome would continue adding amino acids until it hit a stop codon or until it ran out of mRNA.