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I have an assignment where we basically have to make a Velocity vs. Substrate plot and find the Km and Vmax values.

We then have to do a double reciprocal plot and find the Km and Vmax from that.

My first question is if I have to convert the units of my axes so they are uniform.

The substrate is in micromolar while the Velocity is in micromolar/min. I asked my TA and he said no....but I just wanted to double check.

On the double reciprocal plot, I plotted the points on deltagraph and they gave me a straight line for the double reciprocal plot in the form of y=mx+b

Is KM the same as Ks in this case? I was thinking that I could get the value of Km but having the slope equal to Ks/Vmax. I can read Vmax off the graph and solve for Ks which I would determine it to be equal to KM. Is that a correct assumption?

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# Homework Help: Help with Kinetics-double reciprocal plot

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