Hi, I have an assignment where we basically have to make a Velocity vs. Substrate plot and find the Km and Vmax values. We then have to do a double reciprocal plot and find the Km and Vmax from that. My first question is if I have to convert the units of my axes so they are uniform. The substrate is in micromolar while the Velocity is in micromolar/min. I asked my TA and he said no....but I just wanted to double check. On the double reciprocal plot, I plotted the points on deltagraph and they gave me a straight line for the double reciprocal plot in the form of y=mx+b Is KM the same as Ks in this case? I was thinking that I could get the value of Km but having the slope equal to Ks/Vmax. I can read Vmax off the graph and solve for Ks which I would determine it to be equal to KM. Is that a correct assumption?