Hey guys! Really need some help... Not sure if anyone is familiar with this, but I performed a fumurate/malate reaction, one with and one without succinate as an inhibitor. However, after performing the calculations and then attempting to plot a lineweaver-burke plot i got the following results! substrate 0.01 0.006 0.004 0.003 0.002 0.001 1/substrate 100 167 250 333 500 1000 1/velocity + inhibitor 5.34 11.78 16.91 26.21 36.13 45.69 1/v no inhibitor 3.10 3.268 3.356 3.876 4.098 6.329 See my surprise! the final one shows a huge difference! I realise taking the reciprocal poses exaggerates differences... but do these results seem plausible? Should they be that high numbers for 1/v+inhibitor? I would really appreciate any help, I'm not sure where to go from here... I could claim human errors in the experiment, but I don't know if these are wrong... Thanks.