What Sub-Structure Does a 369.3 Mass Indicate in Cholesterol LCMS Analysis?

  • Thread starter Thread starter cdoss
  • Start date Start date
  • Tags Tags
    Assembly
Click For Summary

Discussion Overview

The discussion revolves around the interpretation of a mass observed in LCMS analysis, specifically focusing on a mass of 369.3 and its relation to the structure or sub-structure of cholesterol. Participants explore the implications of this mass in the context of their homework assignment.

Discussion Character

  • Homework-related
  • Exploratory

Main Points Raised

  • Some participants express uncertainty about how to determine the structure or sub-structure corresponding to the observed mass of 369.3 in the LCMS result.
  • One participant notes that the molecular mass of cholesterol is 387.3 and suggests that the LCMS result may reflect a mass that is 18 less due to the breaking of a bond with water.
  • Another participant agrees with the interpretation that the observed mass could be influenced by the addition of a proton in the LCMS analysis.

Areas of Agreement / Disagreement

There is some agreement on the interpretation of the mass observed in the LCMS analysis, particularly regarding the potential influence of water and the addition of a proton. However, uncertainty remains about how to definitively determine the structure or sub-structure associated with the mass of 369.3.

Contextual Notes

Participants do not clarify the assumptions regarding the conditions of the LCMS analysis or the specific structural modifications being considered. The discussion lacks resolution on the exact implications of the observed mass.

cdoss
Messages
8
Reaction score
0

Homework Statement


Our assignment was to determine the structure, or sub-structure of our mass observed in the LCMS result. The number we got was 369.3. I am not really sure what to do with this at all. She gave us the structure of cholesterol, but I'm not sure where to add things on...
 
Physics news on Phys.org
cdoss said:

Homework Statement


Our assignment was to determine the structure, or sub-structure of our mass observed in the LCMS result. The number we got was 369.3. I am not really sure what to do with this at all. She gave us the structure of cholesterol, but I'm not sure where to add things on...

Add things on? Molecular mass of cholesterol is ... what?
 
387.3. I think I actually understand it now. because the LCMS will show the mass +1 and then the bond with water was broken, which caused the weight to be 18 less than what we expected.?
 
cdoss said:
387.3. I think I actually understand it now. because the LCMS will show the mass +1 and then the bond with water was broken, which caused the weight to be 18 less than what we expected.?

yeah,,, agree,,,!:cool::cool::cool::cool::cool::cool::cool:
 
Last edited by a moderator:

Similar threads

What is the Mass of Sulphur in 71g of Na2SO4?
  • · Replies 5 ·
Replies
5
Views
2K
Is mathematics invented or discovered?
  • · Replies 64 ·
3
Replies
64
Views
3K
Calculate mass of NaHCO3 when losing CO2 in experiement
  • · Replies 4 ·
Replies
4
Views
8K
How would I find the volume of the solution?
  • · Replies 4 ·
Replies
4
Views
2K
What mass of P4 is required to combine with 0.398 g of Cl2?
  • · Replies 28 ·
Replies
28
Views
3K
How to Estimate the Energy of a Delocalized π Electron in Benzene?
  • · Replies 3 ·
Replies
3
Views
5K
Does the Powder XRD Data Indicate a Face-Centred Cubic Structure?
  • · Replies 5 ·
Replies
5
Views
6K
Finding Empirical Formula of Propenoic Acid
  • · Replies 2 ·
Replies
2
Views
14K
Chemistry Molecular structure and analysis method
  • · Replies 4 ·
Replies
4
Views
4K
Admissions Could I please get critique on my SOP? (Experimental Biophysics)
  • · Replies 9 ·
Replies
9
Views
2K