Southern Blotting

[nokia8650]

In southern blotting, how are the DNA bands visualised? i have read two conflicting methods; one using ethidium bromide stain, and one using DNA probes. Can either be used, or are the two distinct methods used in different circumstances?

Thanks.

[atyy]

EtBr is just to visualise the DNA, and is non-specific for all DNA. The DNA probe allows you to detect a specific complementary base pair sequence.

[nokia8650]

Thanks so much for the reply; thats was what I thought logically made sense but I wanted to confirm it!