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CDNA to protein sequence?

by gravenewworld
Tags: cdna, protein, sequence
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gravenewworld
#1
Feb14-13, 05:59 PM
P: 1,408
Has anyone here done this before? If so, kit recommendations and protocol? Any help would be awesome. Thanks!
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Ygggdrasil
#2
Feb14-13, 06:14 PM
Other Sci
Sci Advisor
P: 1,384
What exactly do you want to do? Do you have a cDNA sequence and you want to figure out the protein sequence that it codes for?
gravenewworld
#3
Feb15-13, 08:01 AM
P: 1,408
Yes exactly. Getting the cDNA is easy, I have experience with that for PCR, but instead of doing PCR, I was wondering how you can use your cDNA to get the protein sequence. My PI said it should be easily doable. I'm not sure how though.

Ygggdrasil
#4
Feb15-13, 10:07 AM
Other Sci
Sci Advisor
P: 1,384
CDNA to protein sequence?

Once you have the cDNA sequence, you just need to sequence it (send a sample of the cDNA along with the appropriate primers to a DNA sequencing facility/company). You can the just read the protein sequence from the sequence you get (for example, using an online translation tool such as http://www.bioinformatics.org/sms2/translate.html

Let me know if you want more detail on any of these steps.


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