The Bradford Method is a biochemical assay used to determine the concentration of proteins in a sample. It is based on the principle that the dye Coomassie Brilliant Blue G-250 binds to proteins in an acidic environment, causing a shift in its absorption spectrum.
In the Bradford Method, a known concentration of protein is used as a standard to create a standard curve. The sample is then mixed with the dye and the absorbance is measured. The absorbance is compared to the standard curve to determine the protein concentration in the sample.
The Bradford Method is a quick and simple assay that can be performed in a single tube. It also has a wide linear range and is more sensitive than other methods such as the Lowry or BCA assay.
Yes, the Bradford Method is affected by the presence of detergents, reducing agents, and other chemicals that may interfere with the binding of the dye to proteins. It is also not suitable for samples with low protein concentrations.
No, the Bradford Method is not suitable for all types of proteins. It may give inaccurate results for proteins with high levels of acidic or basic amino acids, such as histidine or arginine. In these cases, other protein assays such as the Lowry or BCA method may be more appropriate.