Recently, my lab has bought a BioRad Turbo Blot Transfer system. It allows for you to do an efficient 7 minute transfer from a minigel to nitrocellulose. The transfer buffer, however is very expensive because you can only purchase it in conjunction with precut nitrocellulose membranes and thick filter paper, which greatly increases the cost. We've attempted to recreate the buffer using our own reagents, but with no luck (the voltage and current are not comparable to transfers using their recipe, or the transfer is inconsistent.). Basically, I was wondering if anyone had any clue as to what might be in this buffer and the proportions? I know this is rather specific, but it's worth a shot.