Chemistry Buffer solutions and electrophoresis in seperating amino acids

  • Thread starter Thread starter Saba
  • Start date Start date
  • Tags Tags
    Chemistry
AI Thread Summary
Gel electrophoresis utilizes electrolytic cells to separate amino acids based on their charge, with positively charged species migrating towards the cathode and negatively charged species moving towards the anode. The application of voltage is crucial in this process, as it facilitates the movement of charged particles through the gel medium. This method allows for effective separation and analysis of amino acids in biochemical studies. Understanding the principles of electrophoresis is essential for applications in molecular biology and biochemistry. The discussion highlights the importance of voltage in driving the electrophoretic process.
Saba
Messages
10
Reaction score
2
Homework Statement
Hi, I have posted the question, the answer to the question as well as the data needed for PI. My concern is why do they say anode is a positive terminal here? is anode always the positive terminal and cathode negative terminal in electrophorisis?

Thanks!
Relevant Equations
NA
Screenshot 2024-10-25 at 5.17.38 PM.png
 
Physics news on Phys.org
From Wikipedia article on Gel Electrophoresis:
"Species that are net positively charged will migrate towards the cathode which is negatively charged (because this is an electrolytic rather than galvanic cell), whereas species that are net negatively charged will migrate towards the positively charged anode."
 
mjc123 said:
From Wikipedia article on Gel Electrophoresis:
"Species that are net positively charged will migrate towards the cathode which is negatively charged (because this is an electrolytic rather than galvanic cell), whereas species that are net negatively charged will migrate towards the positively charged anode."
ohh so they use electrolytic cells in electropherisis. I didn't know that thank you.
 
Saba said:
ohh so they use electrolytic cells in electropherisis. I didn't know that thank you.
A voltage is applied -> electrolytic
A voltage is supplied -> electrochemical

Roughly speaking.
 

Thread 'Prove that evolution is constant (Provide at least two arguments to support your position)'

I don't get how to argue it. i can prove: evolution is the ability to adapt, whether it's progression or regression from some point of view, so if evolution is not constant then animal generations couldn`t stay alive for a big amount of time because when climate is changing this generations die. but they dont. so evolution is constant. but its not an argument, right? how to fing arguments when i only prove it.. analytically, i guess it called that (this is indirectly related to biology, im...
View full post »

Similar threads

Chemistry Why Should the Mole Ratio in Alkaline Buffers Be 5:1 Base to Acid?
Replies
3
Views
3K
Chemistry Book seems to say that we can have weak acid and weak conjugate base
Replies
2
Views
1K
Chemistry Understanding how to calculate pH of a buffer solution
Replies
2
Views
1K
Chemistry Moles of NaOH to Create Buffer Solution pH=6 in 0.42M Ethanoic Acid
Replies
3
Views
3K
Chemistry Estimating number of amino acids with protein molecular mass
Replies
1
Views
3K
How many amino acid residues are there for the transmembrane domain?
Replies
1
Views
2K
Chemistry Are there typos in this snippet causing confusion?
Replies
2
Views
1K
Creating a buffer out of 0.100M solutions
Replies
1
Views
2K
Chemistry Why is HCl an Arrhenius acid but hydrogen carbonate isn't?
Replies
4
Views
1K
Chemistry Solving pH Changes: Adding Acid to a Buffer or Water
Replies
16
Views
3K

Hot Threads

Recent Insights

Back
Top