Hi Mango12,
When a particle of charge $q$ is accelerated by an electric field $E$, the electric force is $F=qE$.
When traversing a distance $\Delta x$ the work done is $W = F\Delta x$ (if the force is constant).
That work is equal to the kinetic energy $U_k = \frac 12 m v^2$ that the particle gains (assuming it starts from a speed of about zero).
So we have:
$$W = U_k \quad\Rightarrow\quad F\Delta x = \frac 12 m v^2
\quad\Rightarrow\quad qE\Delta x =\frac 12 m v^2
\quad\Rightarrow\quad qV =\frac 12 m v^2
\quad\Rightarrow\quad V =\frac {m v^2}{2q}
$$