Dr. Nick
- 28
- 0
I’ve really lost my nerves on this one :(, I have to learn some methods and differences between them, but I can’t manage it. And all I have is an internet as help.
Methods are :
FISH
mFISH
Chromosome painting
BandFISH
SKY
I just can’t establish difference between some of them, purposes.
Especially Chromosome painting, c’mon I don’t get it, one site says it’s basically mFISH, on other something else.
This is where I am now:
FISH - using singlestrain DNA fluorescence labeled probe (1 probe I guess)
mFISH - using multiple singlestrain DNA fluorescence labeled probes giving different colours
Chromosome painting – same as mFISH ?
BandFISH – painting whole chromosome in different color bands
SKY – painting all chromosomes (with what ?, DNA probes ?) in different colors for cariotyping
And when in FISH picture you see red probe on chromosome you can see whole chromosome painted in blue, is this done by probes of some kind of color specificlu binding for chromosomes (I’ve saw this in other methods too).
Thank You !
Methods are :
FISH
mFISH
Chromosome painting
BandFISH
SKY
I just can’t establish difference between some of them, purposes.
Especially Chromosome painting, c’mon I don’t get it, one site says it’s basically mFISH, on other something else.
This is where I am now:
FISH - using singlestrain DNA fluorescence labeled probe (1 probe I guess)
mFISH - using multiple singlestrain DNA fluorescence labeled probes giving different colours
Chromosome painting – same as mFISH ?
BandFISH – painting whole chromosome in different color bands
SKY – painting all chromosomes (with what ?, DNA probes ?) in different colors for cariotyping
And when in FISH picture you see red probe on chromosome you can see whole chromosome painted in blue, is this done by probes of some kind of color specificlu binding for chromosomes (I’ve saw this in other methods too).
Thank You !