Estimating number of amino acids with protein molecular mass

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SUMMARY

The discussion centers on estimating the number of amino acids in a protein with a molecular mass of 65,000 g/mol. The correct approach involves dividing the protein's molecular weight by the average weight of an amino acid, which is approximately 110 Da. This calculation yields an estimate of about 590 amino acids. Participants confirm that the average molecular weight of amino acids can be easily found in textbooks or online resources.

PREREQUISITES
  • Understanding of molecular mass and its units (g/mol)
  • Basic knowledge of amino acid structure and average molecular weight (110 Da)
  • Familiarity with protein composition and estimation techniques
  • Access to biochemical textbooks or online databases for molecular weights
NEXT STEPS
  • Research the average molecular weight of different amino acids
  • Learn about protein structure and composition analysis techniques
  • Explore bioinformatics tools for protein mass calculations
  • Study methods for estimating protein size from molecular mass
USEFUL FOR

This discussion is beneficial for students in biochemistry, molecular biology researchers, and educators teaching protein structure and estimation methods.

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Homework Statement


Suppose you were told you had a protein with molecular mass of 65,000 g/mol. Show how you would estimate the number of amino acids present in this protein. Keep in mind that you cannot calculate the exact number, the point of this is to approximate how many amino acids are present.

Homework Equations


I think I would need to divide the protein's weight by the average weigh of its amino acids, right? Problem is how am I suppose to do this when no average weight for amino acids is given in the problem?

The Attempt at a Solution


I just divided 65000 Da by 110 Da (googled average weight of an amino acid) and got about 590 amino acids in the protein. I am completely unsure if this is correct as my lab never discussed this at all while doing bio-spectroscopy. Help would be appreciated.
 
Last edited:
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Yes, that is the correct approach. Your instructor likely expected you to look up the average MW of an amino acid as it is fairly easily found either in a textbook or through Google.
 

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