Identifying Unknown Bacteria: A Scientific Approach

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SUMMARY

The discussion centers on identifying an unknown Gram-negative cocci bacterium that is catalase-positive, oxidase-negative, and has endospores, as determined by the Schaeffer-Fulton method. The bacterium shows resistance to Streptomycin, Chloramphenicol, Penicillin, and Kanamycin. Participants suggest that while Staphylococcus is a possibility due to antibiotic resistance, it is unlikely because Staphylococcus is Gram-positive and does not form endospores. The conversation emphasizes the need for further testing and consultation with microbiology experts for accurate identification.

PREREQUISITES
  • Understanding of Gram staining techniques
  • Familiarity with bacterial morphology, specifically cocci
  • Knowledge of the Schaeffer-Fulton method for endospore staining
  • Basic principles of antibiotic resistance in bacteria
NEXT STEPS
  • Research the characteristics of Gram-negative cocci bacteria
  • Learn about the Schaeffer-Fulton method in detail
  • Investigate the implications of antibiotic resistance patterns
  • Explore methods for consulting with microbiology experts for bacterial identification
USEFUL FOR

This discussion is beneficial for microbiologists, laboratory technicians, and students studying bacteriology, particularly those involved in identifying and classifying unknown bacterial strains.

Chiral_kun
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Homework Statement


I have done tests on this unknown bacteria and this were the results:

Cocci shape Gram (-) negative

Catalase (+) positive (only one bubble though)

Oxidase( - )negative

Has endospores (used Schaeffer-Fulton method)

Antibiogram:

Resistant to Streptomycin, Chloramphemicol, Penicillin and Kanamycin

Homework Equations


N/A

The Attempt at a Solution


Is it Staphylococcus? I searched and the antibiotic resistance lines up. Staphylococcus doesn't form endospores though and is gram positive.
 

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It's been a (very) long time since I did microbiology, but aren't most cocci gram-positive? I think that should narrow it down quite a bit. Also, most endospore-forming bacteria are gram-positive too. So maybe redo some of the tests?
 
Well from what the comments and you are saying we have 2
Possibilities here 1.its staph but a new strain and you get to name it or 2.its a new species you get to name it .i’d Say send some to an expert but if you think you can do it then I believe in you but don't be dissapointed and you can’t figure it out
 

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