# Immobilization of Beta-glucosidase with glutaraldehyde

1. Aug 6, 2015

### Lindsayyyy

Hello everyone,

I want to immobilize beta-glucosidase (on Sepabeads with an amine group). The problem I have is I don't have a strong chemical/biological background. The immobilization should happen with glutaraldehyde but I can't find an appropiate paper with a protocl I can use. In every paper I found there are always insufficient information for me in order to reproduce it.
Does anyone have a good protocol or a paper where I can find something about this? I'm quite desperate at the moment because I've been trying to immobilize this enzyme for quite some time now without any success.