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skisci
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Are there any methods to "knockout" genes in-vivo? For example, the HOXB7 gene (http://www.physorg.com/news/2010-12-hoxb7-gene-tamoxifen-resistance.html) in cancer patents.
skisci said:Are there any methods to "knockout" genes in-vivo? For example, the HOXB7 gene (http://www.physorg.com/news/2010-12-hoxb7-gene-tamoxifen-resistance.html) in cancer patents.
skisci said:Thanks.
Is there any leakage from unbinded mRNA fragments that then do get translated into proteins?
Could one tailor a virus to splice the siRNA DNA into a target cell so the siRNA could be manufactured inter-cellularly? (I.E. Lytic cycle without the virus parts)
skisci said:siRNA, from my understanding, can interfere with gene expression post-transcriptionally and therefore can be used on an unprimed (if you will) genome. I feel this route has more promise for clinical applications. A few questions.
Andy Resnick said:Gene therapy has been pursued for more than a decade, with not much success:
http://www.ornl.gov/sci/techresources/Human_Genome/medicine/genetherapy.shtml
I'm (somewhat) familiar with the case of Jesse Gelsinger. He had a variant of cystic fibrosis, which was identified as a candidate disease for gene therapy since the lung is an easy organ to target (inhale the carrier).
People are still trying various approaches with some success, but progress is very slow.
skisci said:Is there any leakage from unbinded mRNA fragments that then do get translated into proteins?
Could one tailor a virus to splice the siRNA DNA into a target cell so the siRNA could be manufactured inter-cellularly? (I.E. Lytic cycle without the virus parts)
In-vivo gene silencing is a process in which specific genes are intentionally deactivated or "knocked out" in living organisms. This is typically done for research purposes to study the effects of the gene's absence on the organism's physiology and behavior.
In-vivo gene silencing is achieved through the use of various techniques, such as RNA interference (RNAi), CRISPR-Cas9, and antisense oligonucleotides. These methods target specific genes and disrupt their expression, effectively silencing them.
In-vivo gene silencing has a wide range of potential applications, including studying gene function, identifying potential drug targets, and developing gene therapies for genetic disorders. It can also be used as a tool to better understand disease mechanisms and develop treatments.
In-vivo gene silencing offers several advantages over traditional gene knockout methods, such as being less time-consuming and cost-effective. It also allows for the selective silencing of specific genes, rather than the complete deletion of the gene, which can have unintended consequences.
There are ethical considerations surrounding in-vivo gene silencing, particularly when it comes to using it in humans. Some concerns include the potential for unintended effects on other genes and the potential for misuse or abuse of the technology. It is important for researchers to carefully consider these ethical implications and adhere to strict regulations and guidelines when conducting in-vivo gene silencing experiments.