Is a smeared band on a Western Blot a valid result?

  • Biology
  • Thread starter Sunwoo Bae
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    Protein
  • #1
Sunwoo Bae
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Homework Statement
I am trying to analyze a western blot result of expression of Keratin Sulfate in variety of cells.
Relevant Equations
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1703601896915.png

From this result, the conclusion is that MDCK-siNGT and MDCK-siB4Gal4 resulted in less keratin sulfate, while MDCK-RCA did not produce keratin sulfate at all. Looking at the gel, the band look very smeared, as if nothing was purified prior to blotting. I have noticed a similar looking gel in other articles as well, but is this a valid result?
 
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