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victorqed
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Hello
I'm trying to implement a application that will scan the electrophoresis gels and draw a graphic of the proteins: albumin, alpha1, alpha2, beta1, beta2 and gamma.
The problem is that the resulted graphic is not as it should be. Albumin percentage is too low and the rest of the proteins are too high. Also, looking at the my graphic I can see that albumin is very low.
I tried to make a comparative test with many applications that already exist on the web and all of them are giving me the same values as I calculated. But this values are wrong so it is clear that I'm missing something essential.
One of this applications is ImageJ(open source).
http://imagej.nih.gov/ij/
http://lukemiller.org/index.php/2010/11/analyzing-gels-and-western-blots-with-image-j/
Example:
I have attached a scan of a normal control done on a Sebia gel.
Correct values are:
Albumin : 66,05%
Alpha1 : 2,47%
Alpha2 : 9,14%
Beta1 : 6,9%
Beta2 : 4,33%
Gamma : 11,11%
Values obtained with my app and ImageJ are:
Albumin: 46,76%
Alpha1 : 3,77%
Alpha2 : 15,05%
Beta1 : 11,78%
Beta2 : 5,36%
Gamma : 17,29%
How can I reproduce the correct values?
Thank you
I'm trying to implement a application that will scan the electrophoresis gels and draw a graphic of the proteins: albumin, alpha1, alpha2, beta1, beta2 and gamma.
The problem is that the resulted graphic is not as it should be. Albumin percentage is too low and the rest of the proteins are too high. Also, looking at the my graphic I can see that albumin is very low.
I tried to make a comparative test with many applications that already exist on the web and all of them are giving me the same values as I calculated. But this values are wrong so it is clear that I'm missing something essential.
One of this applications is ImageJ(open source).
http://imagej.nih.gov/ij/
http://lukemiller.org/index.php/2010/11/analyzing-gels-and-western-blots-with-image-j/
Example:
I have attached a scan of a normal control done on a Sebia gel.
Correct values are:
Albumin : 66,05%
Alpha1 : 2,47%
Alpha2 : 9,14%
Beta1 : 6,9%
Beta2 : 4,33%
Gamma : 11,11%
Values obtained with my app and ImageJ are:
Albumin: 46,76%
Alpha1 : 3,77%
Alpha2 : 15,05%
Beta1 : 11,78%
Beta2 : 5,36%
Gamma : 17,29%
How can I reproduce the correct values?
Thank you
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