Raman Spectroscopy help

  • Context: Undergrad 
  • Thread starter Thread starter labgirl
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SUMMARY

The discussion centers on the challenges faced when using a Raman TruScan with a 785 nm laser for material identification, specifically regarding excessive fluorescence that hampers signal quality. A contrasting scenario is presented where another lab successfully obtains a strong signal with the same equipment and sample. The key factors influencing the differing results include instrument calibration, sample preparation, and environmental conditions affecting fluorescence levels.

PREREQUISITES
  • Understanding of Raman spectroscopy principles
  • Familiarity with fluorescence interference in spectroscopic analysis
  • Knowledge of instrument calibration techniques
  • Experience with sample preparation methods for Raman analysis
NEXT STEPS
  • Research methods to minimize fluorescence in Raman spectroscopy
  • Learn about Raman TruScan calibration procedures
  • Explore sample preparation techniques for optimal Raman signal
  • Investigate environmental factors affecting Raman spectroscopy results
USEFUL FOR

Researchers and technicians in analytical chemistry, particularly those working with Raman spectroscopy for material identification and analysis.

labgirl
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TL;DR
Two raman instruments of the same model not giving reproducible results
I am attempting to use a Raman TruScan with a 785 nm laser to read a material for identification purposes. The material causes too much fluorescence and doesn’t not produce a good signal. However another lab is able to produce a good signal consistently using the same Raman model and sample material. What would be the reason for the different results between instruments?
 
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