The regiment flew harassment bombing and precision bombing[2] missions from 1942 to the end of the war. At its largest size, it had 40 two-person crews. It flew over 23,000 sorties and is said to have dropped 3,000 tons of bombs. It was the most highly-decorated unit in the Soviet Air Force, each pilot having flown over 1,000 missions by the end of the war and twenty-three having been awarded the Hero of the Soviet Union title. Thirty-one of its members died in combat.
The regiment flew in wood and canvas Polikarpov Po-2 biplanes, a 1928 design intended for use as training aircraft and for crop-dusting. The planes could carry only two bombs at a time, so multiple missions in a night were necessary. Although the aircraft were obsolete and slow, the pilots made daring use of their exceptional maneuverability; they had the advantage of having a maximum speed that was lower than the stall speed of both the Messerschmitt Bf 109 and the Focke-Wulf Fw 190, as a result, the German pilots found them very difficult to shoot down. A stealthiness technique of the night bombers was to idle the engine near the target and glide to the bomb release point, with only wind noise to reveal their location.