well the tangent space to R^n at any point is uniquely R^n, and this says that any chart carries this one unique model tangent space isomorphically to yours. so yours is determined by this. i.e. the unique tangent space to your manifold is whatever space R^n maps to by (the derivative of) any chart.
It is really not clear what is being asked for here. In calculus we define the tangent line as a certain limit, provided that limiting line is unique, i.e. the slope is defined as a certain limit provided that limit "exists", which means it is the unique accumulation point of our secant slopes. .so in calculus, tangent lines are unique by definition.
If you allow any limiting value of such secant slopes then for a figure eight curve you can get more than one tangent line, and the conclusion is that your figure eight curve is not a smooth manifold at the point. so in some sense, an embedded variety is a manifold if and only if its tangent space is unique.
if you consider an abstract manifold, then it has a definition for its tangent space, and if you accept that definition and if that definition specifies only one space, then again uniqueness is part of the definition. But as stated, the tangent space is not unique since the definitions are not unique. i.e. there are many equivalent definitions of a tangent space, and so uniqueness can only mean that they are compatible in the sense that any diffeomorphism of manifolds maps one definition to the other.
i.e. a tangent bundle construction is a functor that assigns to each manifold a vector bundle with certain properties: namely the tangent bundle of R^n is R^n x R^n, and any smooth map of manifolds induces a bundle map, and in the case of R^n that bundle map is given by the matrix of partial derivatives, and given any open subset of a manifold, the tangent bundle of that subset is the restriction to that subset of the tangent bundle on the big manifold. If you look in Spivak's differential geometry book vol 1, you will see this abstract functorial development characterizing the tangent bundle construction.
so before this question can be answered you have to make the question precise somehow. but for subsets of the plane, say plane curves, the usual definition makes sense and it is interesting to characterize those curves and those points at which the tangent line is in fact unique, and this is equivalent to plane curves which are either smooth manifolds at the point, or more generally have only one "branch" at the given point. e.g. the curve y^2 = x^3 is not a manifold at the origin but there is still only one tangent line there, the x axis. nonetheless, with the correct definition, (not just limiting positions of lines obtained from secants, but as the zero locus of the linear term of the defining polynomial), the tangent space at that point is actually 2 dimensional, which implies the curve is not a smooth submanifold of the plane at that point.