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garytse86
Jan30-04, 01:08 PM
I am currently working on an investigation on the effects of varying enzyme concentration on the rate of hydrolysis of casein. I have looked on the internet to work out the maximum rate, but they all depnd on one variable - substrate concentration. I cannot find any mathematical expressions to work out rate by altering the concneration of enzyme. Can anyone help please??

Monique
Jan30-04, 01:17 PM
How about the Michaelis-Menten equation?

V=\frac{k_2[E]_t[S]}{K_M+[S]}

garytse86
Feb3-04, 07:00 AM
thank you very much